Journal of Clinical and Molecular Endocrinology

In Utero Exposure of Biochanin-A Alters Female Reproduction in Rat.

Biochanin-A (BCA) is an isoflavone chemically called as 4-methyl genistein. It is plant derived non-steroidal compound, possesses estrogen like biological Activity The present study focussed on the changes of Reproduction in female rats exposed to BCA during the perinatal period.Pregnant rats were intra peritoneally exposed to 25 mg, 50 mg and 100 mg/kg body weight (wt.) on gestation days 12th, 14th, 16th and 18th. Rats were allowed to deliver pups and the BCA exposure was continued lactationally till weaning. Female pups were separated and fed with normal pellet for 80 days and then estrus cycle length was measured. Significanƚ increase in the duration of the estrus cycle was measured in rats exposed to BCA during the perinatal period. To assess the Reproductive performance of BCA exposed female progeny, mated with 90days old normal males. Observed increase in the Conception time and decrease in the number of implantations and also increase in pre and post implantations loss. To confirm the BC-A induced Reproductive Abnormalties in females computatinally docking studies was performed with aromatase and β estrogen receptor (β-ER) and in comparison with its substrates androstenedione and estradiol respectively along with BC-A and found strong competitive inhibition by BC-A with the substrates of aromatase and β-ER. It is very clear from the result that pre and post natal exposure of BCA suppresses the female reproduction in rats..

Author(s):

M.G.S. Soujanya, K. Prathap Reddy, V. Sridevi, P. Ramachandra Reddy and P.Sreenivasula Reddy

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